Molecular Characterization of Growth Hormone Gene in Exotic and Crossbred Pigs
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چکیده
Growth hormone (GH) plays a central role in growth and production through its influence on important metabolic activities in mammals which prompted its wide commercial use including use as a candidate marker. In present experiment, polymorphism at exon 1 and 2 of Growth Hormone gene (GH) was studied using PCR– RFLP in crossbred and exotic pigs, commonly found in north east India. Restriction enzyme digestion of 607 bp PCR amplicon with ApaI, HaeII, DdeI and MspI revealed 3, 3, 3 and 1 genotyopes, respectively. Comparison of obtained sequences revealed large scale nucleotide and residue substitutions. Observed polymorphism may be associated with wide variability in growth (rate) of different breeds of pig.
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تاریخ انتشار 2014